METHYLATION ASSAY - AN OVERVIEW

The procedure of HPLC-UV (high general performance liquid chromatography-ultraviolet), designed by Kuo and colleagues in 1980 [17], remains thought to be the current “gold standard” assay for quantifying the quantity of deoxycytidine (dC) and methylated cytosines (5 mC) present within a hydrolysed DNA sample. However, the utility of this techni